Analyses are done with a Biochrom 30 amino acid analyzer. Taurine in plasma, urine, tissue and feed: the sample preparation procedure is the same as complete amino acid analysis. Gaithersburg, Md: AOAC International, 2005 88–89. In: Official methods of analysis of AOAC International. To analyze for tryptophan in foods and food and feed ingredients, we use AOAC official method 988.15.Gaithersburg, Md: AOAC International, 2005 9–19. We use the following method for amino acids : Amino acids in feeds. Note: An additional run is necessary to analyze feed samples for methionine or tryptophan. We Process Samples using the Following MethodsĬomplete amino acids in physiological fluid samples: add 6% Sulfosalicylic Acid (SSA) (1:1) to sample for deproteinization, centrifuge the mixture at 14000 rm for 25 minutes, filter the supernatant through 0.45 mm syringe drive PTFE filter, adjust pH to 2.2, load 50 ml on Biochrom 30 amino acid analyzer.Ĭomplete amino acids in solid samples: hydrolyze 5 mg sample using 5 ml of 6 Nmol HCl in sealed ampoule (110✬, 24 hrs.), dry the sample with nitrogen gas, dissolve it again in loading buffer, filter the hydrolyte and load 50 ml on Biochrom 30 amino acid analyzer without dilution.įree amino acids in tissues or feed: add 3% SSA solution to homogenized sample (10:1 V:W), let stand overnight at room temperature, centrifuge and filter the supernatant (0.45mm), adjust pH to 2.2 and load 50 ml on Biochrom 30 amino acid analyzer. Please send at least 1 ml in a urine tube (white top) or other “no additives” tube.Urine (for complete amino acid analysis or taurine) Remove the needle and place the blood in a no additives tube (i.e.: white top, red top, but NOT a serum separator). Draw blood into the syringe followed by approximately 1cc of air to mix the blood and heparin thoroughly (invert approximately 5 times). If no green top is available: Add 2-3 drops of heparin in a syringe.If using a lithium-heparin (green top) tube: Draw 2 ml (or more) of blood into the green top tube (not outdated).Heparinized plasma should be straw colored and not hemolyzed. Put the heparinized plasma into a no-additives tube (i.e.: white top, red top, but NOT a serum separator). Take care not to disturb the buffy coat (white blood cells contain a lot of taurine). Immediately centrifuge the blood and take off the plasma using a Pasteur pipette or eye dropper. PLEASE NOTE: we do NOT spin down samples for plasma in our laboratory, if a plasma test is desired, you must send us the sample as plasma Plasma (for complete amino acid analysis or taurine)
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